Servio H Ramirez

Servio H Ramirez,

Mary And Bryan Whisenant Distinguished Professor

Department: MD-PATHOLOGY
Business Email: ramirez.servio@ufl.edu

About Servio H Ramirez

Accomplishments

Mary and Bryan Whisenant Distinguished Professor of Pathology
2023-Current · University of Florida, College of Medicine, Gainesville, FL
Tenured Professor, Department of Pathology & Laboratory Medicine,
2020-2023 · The Lewis Katz School of Medicine at Temple University, Philadelphia, PA
Associate Professor, Department of Pathology & Laboratory Medicine
2014-2020 · The Lewis Katz School of Medicine at Temple University, Philadelphia PA
Director, Histopathology Research Core
2011-2023 · The Lewis Katz School of Medicine at Temple University, Department of Pathology & Laboratory Medicine, Philadelphia, PA
Assistant Professor, Department of Pathology & Laboratory Medicine
2008-2014 · The Lewis Katz School of Medicine at Temple University, Philadelphia PA

Teaching Profile

Teaching Philosophy
To prospective students looking to join the laboratory: We teach a scientific framework in which students are encouraged to expand and not restrict their way of thinking. We promote the challenging of existing dogmas and scrutinizing of indirectly derived assumptions about biological phenomena. Students will be helped to study newly evolving scientific trends and to also keep current on relevant published scientific information. We believe in a scientific practice that is both independent and highly cooperative. Our experience has provided us with the benchmarks necessary to ensure effective training in the: formulation of testable hypotheses, technical proficiency, critically analysis of results and communication of scientific findings. Please contact us to learn more about currently available research opportunities.

Research Profile

The laboratory focuses on the study of how brain pathologies affect the neurovascular unit (NVU). Given that the blood brain barrier (BBB) is the gateway to the CNS, we approach our studies by centralizing our efforts around this highly specialized structure. The ongoing research can be categorized as follows: 1) assay development to usher-in the next generation of microfluidic modeling of the neurovascular unit, 2) characterization of the temporal and spatial progression of neuroinflammation, 3) serological biomarker discovery for evaluation of BBB damage during brain injury, and 4) intervention with pharmacological or biological agents that can halt damage or enhance repair of NVUs. Our expertise is interdisciplinary and meets at the intersection of neuroscience, immunology, molecular biology, microbiology, translational medicine, pharmacology and bioengineering.

Areas of Interest
  • Cerebral Vascular Biology
  • Infectious diseases of the CNS
  • Neuroinflammation
  • Serological Biomarkers
  • Tissue engineering
  • Traumatic Brain Injury (TBI)

Publications

2023
A Next-Generation 3D Tissue-Engineered Model of the Human Brain Microvasculature to Study the Blood-Brain Barrier.
Bioengineering (Basel, Switzerland). 10(7) [DOI] 10.3390/bioengineering10070817. [PMID] 37508844.
2023
An Engineered Adeno-Associated Virus Capsid Mediates Efficient Transduction of Pericytes and Smooth Muscle Cells of the Brain Vasculature.
Human gene therapy. 34(15-16):682-696 [DOI] 10.1089/hum.2022.211. [PMID] 37376759.
2023
Concussions in young adult athletes: No effect on cerebral white matter.
Frontiers in human neuroscience. 17 [DOI] 10.3389/fnhum.2023.1113971. [PMID] 36936617.
2023
Retraction Note: VPS35 regulates tau phosphorylation and neuropathology in tauopathy.
Molecular psychiatry. 28(4) [DOI] 10.1038/s41380-023-01973-9. [PMID] 36697754.
2021
Correction to: Extracellular Microvesicles Released From Brain Endothelial Cells are Detected in Animal Models Of HIV‑1 Signifying Unresolved Inflammation.
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology. 16(4):888-889 [DOI] 10.1007/s11481-021-10024-5. [PMID] 34596820.
2021
ErbB3 is a critical regulator of cytoskeletal dynamics in brain microvascular endothelial cells: Implications for vascular remodeling and blood brain barrier modulation.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism. 41(9):2242-2255 [DOI] 10.1177/0271678X20984976. [PMID] 33583260.
2021
Extracellular Microvesicles Released From Brain Endothelial Cells are Detected in Animal Models Of HIV-1 Signifying Unresolved Inflammation.
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology. 16(4):785-795 [DOI] 10.1007/s11481-021-10008-5. [PMID] 34435263.
2021
SARS-CoV-2 Spike Protein Disrupts Blood-Brain Barrier Integrity via RhoA Activation.
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology. 16(4):722-728 [DOI] 10.1007/s11481-021-10029-0. [PMID] 34687399.
2021
The psychoactive drug of abuse mephedrone differentially disrupts blood-brain barrier properties.
Journal of neuroinflammation. 18(1) [DOI] 10.1186/s12974-021-02116-z. [PMID] 33648543.
2021
The Use of Tissue Engineering to Fabricate Perfusable 3D Brain Microvessels in vitro.
Frontiers in physiology. 12 [DOI] 10.3389/fphys.2021.715431. [PMID] 34531761.
2021
VPS35 regulates tau phosphorylation and neuropathology in tauopathy.
Molecular psychiatry. 26(11):6992-7005 [DOI] 10.1038/s41380-019-0453-x. [PMID] 31289348.
2020
Experimental Traumatic Brain Injury during Adolescence Enhances Cocaine Rewarding Efficacy and Dysregulates Dopamine and Neuroimmune Systems in Brain Reward Substrates.
Journal of neurotrauma. 37(1):27-42 [DOI] 10.1089/neu.2019.6472. [PMID] 31347447.
2020
HIV infects astrocytes in vivo and egresses from the brain to the periphery.
PLoS pathogens. 16(6) [DOI] 10.1371/journal.ppat.1008381. [PMID] 32525948.
2020
Reward and immune responses in adolescent females following experimental traumatic brain injury.
Behavioural brain research. 379 [DOI] 10.1016/j.bbr.2019.112333. [PMID] 31682867.
2020
The SARS-CoV-2 spike protein alters barrier function in 2D static and 3D microfluidic in vitro models of the human blood-brain barrier.
bioRxiv : the preprint server for biology. [DOI] 10.1101/2020.06.15.150912. [PMID] 32587958.
2020
The SARS-CoV-2 spike protein alters barrier function in 2D static and 3D microfluidic in-vitro models of the human blood-brain barrier.
Neurobiology of disease. 146 [DOI] 10.1016/j.nbd.2020.105131. [PMID] 33053430.
2019
Blockade of MCU-Mediated Ca2+ Uptake Perturbs Lipid Metabolism via PP4-Dependent AMPK Dephosphorylation.
Cell reports. 26(13):3709-3725.e7 [DOI] 10.1016/j.celrep.2019.02.107. [PMID] 30917323.
2019
Brain interrupted: Early life traumatic brain injury and addiction vulnerability.
Experimental neurology. 317:191-201 [DOI] 10.1016/j.expneurol.2019.03.003. [PMID] 30862466.
2019
Characterization of cancer-associated IDH2 mutations that differ in tumorigenicity, chemosensitivity and 2-hydroxyglutarate production.
Oncotarget. 10(28):2675-2692 [DOI] 10.18632/oncotarget.26848. [PMID] 31105869.
2019
Endothelial Targeted Strategies to Combat Oxidative Stress: Improving Outcomes in Traumatic Brain Injury.
Frontiers in neurology. 10 [DOI] 10.3389/fneur.2019.00582. [PMID] 31275220.
2019
Selection of an Efficient AAV Vector for Robust CNS Transgene Expression.
Molecular therapy. Methods & clinical development. 15:320-332 [DOI] 10.1016/j.omtm.2019.10.007. [PMID] 31788496.
2019
Sex-specific neurogenic deficits and neurocognitive disorders in middle-aged HIV-1 Tg26 transgenic mice.
Brain, behavior, and immunity. 80:488-499 [DOI] 10.1016/j.bbi.2019.04.029. [PMID] 30999016.
2018
Characterization of human fetal brain endothelial cells reveals barrier properties suitable for in vitro modeling of the BBB with syngenic co-cultures.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism. 38(5):888-903 [DOI] 10.1177/0271678X17708690. [PMID] 28534661.
2018
Effects of Platelet-Activating Factor on Brain Microvascular Endothelial Cells.
Neuroscience. 377:105-113 [DOI] 10.1016/j.neuroscience.2018.02.039. [PMID] 29522856.
2018
Extracellular vesicles: mediators and biomarkers of pathology along CNS barriers.
Fluids and barriers of the CNS. 15(1) [DOI] 10.1186/s12987-018-0104-7. [PMID] 29960602.
2017
Acute administration of catalase targeted to ICAM-1 attenuates neuropathology in experimental traumatic brain injury.
Scientific reports. 7(1) [DOI] 10.1038/s41598-017-03309-4. [PMID] 28630485.
2017
Adolescent Traumatic Brain Injury Induces Chronic Mesolimbic Neuroinflammation with Concurrent Enhancement in the Rewarding Effects of Cocaine in Mice during Adulthood.
Journal of neurotrauma. 34(1):165-181 [DOI] 10.1089/neu.2015.4275. [PMID] 27026056.
2017
Antibody blockade of CLEC12A delays EAE onset and attenuates disease severity by impairing myeloid cell CNS infiltration and restoring positive immunity.
Scientific reports. 7(1) [DOI] 10.1038/s41598-017-03027-x. [PMID] 28578388.
2017
Dexamethasone Attenuates the Enhanced Rewarding Effects of Cocaine Following Experimental Traumatic Brain Injury.
Cell transplantation. 26(7):1178-1192 [DOI] 10.1177/0963689717714341. [PMID] 28933216.
2017
Factors affecting increased risk for substance use disorders following traumatic brain injury: What we can learn from animal models.
Neuroscience and biobehavioral reviews. 77:209-218 [DOI] 10.1016/j.neubiorev.2017.03.015. [PMID] 28359860.
2017
Trafficking of adeno-associated virus vectors across a model of the blood-brain barrier; a comparative study of transcytosis and transduction using primary human brain endothelial cells.
Journal of neurochemistry. 140(2):216-230 [DOI] 10.1111/jnc.13861. [PMID] 27718541.
2016
Blood biomarkers for brain injury: What are we measuring?
Neuroscience and biobehavioral reviews. 68:460-473 [DOI] 10.1016/j.neubiorev.2016.05.009. [PMID] 27181909.
2016
Dysfunction of brain pericytes in chronic neuroinflammation.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism. 36(4):794-807 [DOI] 10.1177/0271678X15606149. [PMID] 26661157.
2016
Exosome-associated AAV vector as a robust and convenient neuroscience tool.
Gene therapy. 23(4):380-92 [DOI] 10.1038/gt.2016.11. [PMID] 26836117.
2016
Exosome-associated AAV vector as a robust and convenient neuroscience tool.
Gene therapy. 23(11) [DOI] 10.1038/gt.2016.65. [PMID] 27808124.
2016
Mechanical Injury Induces Brain Endothelial-Derived Microvesicle Release: Implications for Cerebral Vascular Injury during Traumatic Brain Injury.
Frontiers in cellular neuroscience. 10 [DOI] 10.3389/fncel.2016.00043. [PMID] 26973460.
2016
Methamphetamine alters microglial immune function through P2X7R signaling.
Journal of neuroinflammation. 13(1) [DOI] 10.1186/s12974-016-0553-3. [PMID] 27117066.
2016
Methamphetamine induces trace amine-associated receptor 1 (TAAR1) expression in human T lymphocytes: role in immunomodulation.
Journal of leukocyte biology. 99(1):213-23 [DOI] 10.1189/jlb.4A0814-395RR. [PMID] 26302754.
2016
Neuregulin1-β decreases interleukin-1β-induced RhoA activation, myosin light chain phosphorylation, and endothelial hyperpermeability.
Journal of neurochemistry. 136(2):250-7 [DOI] 10.1111/jnc.13374. [PMID] 26438054.
2015
Activation of Cannabinoid Type Two Receptors (CB2) Diminish Inflammatory Responses in Macrophages and Brain Endothelium.
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology. 10(2):302-8 [DOI] 10.1007/s11481-015-9591-3. [PMID] 25666933.
2015
Identification and dynamic regulation of tight junction protein expression in human neural stem cells.
Stem cells and development. 24(12):1377-89 [DOI] 10.1089/scd.2014.0497. [PMID] 25892136.
2015
Poly(ADP-ribose) polymerase-1 inhibition in brain endothelium protects the blood-brain barrier under physiologic and neuroinflammatory conditions.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism. 35(1):28-36 [DOI] 10.1038/jcbfm.2014.167. [PMID] 25248836.
2014
Characterization of platelet-monocyte complexes in HIV-1-infected individuals: possible role in HIV-associated neuroinflammation.
Journal of immunology (Baltimore, Md. : 1950). 192(10):4674-84 [DOI] 10.4049/jimmunol.1302318. [PMID] 24729609.
2014
Emerging roles of pericytes in the regulation of the neurovascular unit in health and disease.
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology. 9(5):591-605 [DOI] 10.1007/s11481-014-9557-x. [PMID] 25119834.
2014
Gene therapy for the nervous system: challenges and new strategies.
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics. 11(4):817-39 [DOI] 10.1007/s13311-014-0299-5. [PMID] 25159276.
2013
Anti-inflammatory effect of targeted delivery of SOD to endothelium: mechanism, synergism with NO donors and protective effects in vitro and in vivo.
PloS one. 8(10) [DOI] 10.1371/journal.pone.0077002. [PMID] 24146950.
2013
Attenuation of HIV-1 replication in macrophages by cannabinoid receptor 2 agonists.
Journal of leukocyte biology. 93(5):801-10 [DOI] 10.1189/jlb.1012523. [PMID] 23463725.
2013
Inhibition of glycogen synthase kinase 3β promotes tight junction stability in brain endothelial cells by half-life extension of occludin and claudin-5.
PloS one. 8(2) [DOI] 10.1371/journal.pone.0055972. [PMID] 23418486.
2012
Activation of cannabinoid receptor 2 attenuates leukocyte-endothelial cell interactions and blood-brain barrier dysfunction under inflammatory conditions.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 32(12):4004-16 [DOI] 10.1523/JNEUROSCI.4628-11.2012. [PMID] 22442067.
2012
Glycogen synthase kinase 3β inhibition prevents monocyte migration across brain endothelial cells via Rac1-GTPase suppression and down-regulation of active integrin conformation.
The American journal of pathology. 181(4):1414-25 [DOI] 10.1016/j.ajpath.2012.06.018. [PMID] 22863953.
2012
Tetherin has negligible activity in restricting hepatitis C virus in hepatocytes.
Innate immunity. 18(3):398-405 [DOI] 10.1177/1753425911412984. [PMID] 21940748.
2011
HIV-1 infection and alcohol abuse: neurocognitive impairment, mechanisms of neurodegeneration and therapeutic interventions.
Brain, behavior, and immunity. 25 Suppl 1(Suppl 1):S61-70 [DOI] 10.1016/j.bbi.2011.03.001. [PMID] 21397004.
2010
Angiotensin II induced cerebral microvascular inflammation and increased blood-brain barrier permeability via oxidative stress.
Neuroscience. 171(3):852-8 [DOI] 10.1016/j.neuroscience.2010.09.029. [PMID] 20870012.
2010
Dyad of CD40/CD40 ligand fosters neuroinflammation at the blood-brain barrier and is regulated via JNK signaling: implications for HIV-1 encephalitis.
The Journal of neuroscience : the official journal of the Society for Neuroscience. 30(28):9454-64 [DOI] 10.1523/JNEUROSCI.5796-09.2010. [PMID] 20631174.
2010
Establishment of primary cultures of human brain microvascular endothelial cells to provide an in vitro cellular model of the blood-brain barrier.
Nature protocols. 5(7):1265-72 [DOI] 10.1038/nprot.2010.76. [PMID] 20595955.
2010
Inhibition of glycogen synthase kinase 3beta (GSK3beta) decreases inflammatory responses in brain endothelial cells.
The American journal of pathology. 176(2):881-92 [DOI] 10.2353/ajpath.2010.090671. [PMID] 20056834.
2010
Methamphetamine causes mitrochondrial oxidative damage in human T lymphocytes leading to functional impairment.
Journal of immunology (Baltimore, Md. : 1950). 185(5):2867-76 [DOI] 10.4049/jimmunol.0903691. [PMID] 20668216.
2009
Methamphetamine disrupts blood-brain barrier function by induction of oxidative stress in brain endothelial cells.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism. 29(12):1933-45 [DOI] 10.1038/jcbfm.2009.112. [PMID] 19654589.
2009
Monocyte chemotactic protein-1 regulates voltage-gated K+ channels and macrophage transmigration.
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology. 4(1):47-59 [DOI] 10.1007/s11481-008-9135-1. [PMID] 19034671.
2008
Activation of peroxisome proliferator-activated receptor gamma (PPARgamma) suppresses Rho GTPases in human brain microvascular endothelial cells and inhibits adhesion and transendothelial migration of HIV-1 infected monocytes.
Journal of immunology (Baltimore, Md. : 1950). 180(3):1854-65 [PMID] 18209083.
2008
Activation of protein tyrosine kinases and matrix metalloproteinases causes blood-brain barrier injury: Novel mechanism for neurodegeneration associated with alcohol abuse.
Glia. 56(1):78-88 [PMID] 17943953.
2008
Mechanism of alcohol-induced oxidative stress and neuronal injury.
Free radical biology & medicine. 45(11):1542-50 [DOI] 10.1016/j.freeradbiomed.2008.08.030. [PMID] 18845238.
2008
Peroxisome proliferator-activated receptor-gamma activation suppresses HIV-1 replication in an animal model of encephalitis.
AIDS (London, England). 22(13):1539-49 [DOI] 10.1097/QAD.0b013e3283081e08. [PMID] 18670212.
2008
Phosphorylation of claudin-5 and occludin by rho kinase in brain endothelial cells.
The American journal of pathology. 172(2):521-33 [DOI] 10.2353/ajpath.2008.070076. [PMID] 18187566.
2007
Oxidative stress activates protein tyrosine kinase and matrix metalloproteinases leading to blood-brain barrier dysfunction.
Journal of neurochemistry. 101(2):566-76 [PMID] 17250680.
2007
T cell independent mechanism for copolymer-1-induced neuroprotection.
European journal of immunology. 37(11):3143-54 [PMID] 17948266.
2006
Blood-brain barrier: structural components and function under physiologic and pathologic conditions.
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology. 1(3):223-36 [PMID] 18040800.
2006
Recombinant adenovirus type 5 vectors that target DC-SIGN, ChemR23 and alpha(v)beta3 integrin efficiently transduce human dendritic cells and enhance presentation of vectored antigens.
Vaccine. 24(5):671-82 [PMID] 16154247.
2005
Motor neuron pathology in experimental autoimmune encephalomyelitis: studies in THY1-YFP transgenic mice.
Brain : a journal of neurology. 128(Pt 8):1877-86 [PMID] 15901645.
2005
Valproic acid enhances gene expression from viral gene transfer vectors.
Journal of virological methods. 125(1):23-33 [PMID] 15737413.
2004
Activation of adenosine A2A receptor protects sympathetic neurons against nerve growth factor withdrawal.
Journal of neuroscience research. 77(2):258-69 [PMID] 15211592.
2004
Dishevelled promotes neurite outgrowth in neuronal differentiating neuroblastoma 2A cells, via a DIX-domain dependent pathway.
Brain research. Molecular brain research. 132(1):38-50 [PMID] 15548427.
2004
Mechanism of NF-kappaB inactivation induced by survival signal withdrawal in cerebellar granule neurons.
The European journal of neuroscience. 20(2):345-52 [PMID] 15233744.
2001
Activation of glycogen synthase kinase 3 beta (GSK-3beta) by platelet activating factor mediates migration and cell death in cerebellar granule neurons.
The European journal of neuroscience. 13(10):1913-22 [PMID] 11403684.
2001
Neurotrophins prevent HIV Tat-induced neuronal apoptosis via a nuclear factor-kappaB (NF-kappaB)-dependent mechanism.
Journal of neurochemistry. 78(4):874-89 [PMID] 11520908.
2000
Functional interplay between nuclear factor-kappaB and c-Jun integrated by coactivator p300 determines the survival of nerve growth factor-dependent PC12 cells.
Journal of neurochemistry. 74(2):527-39 [PMID] 10646503.
1999
HIV-1 Tat-mediated activation of glycogen synthase kinase-3beta contributes to Tat-mediated neurotoxicity.
Journal of neurochemistry. 73(2):578-86 [PMID] 10428053.
1999
Mitogenic up-regulation of the PRL-1 protein-tyrosine phosphatase gene by Egr-1. Egr-1 activation is an early event in liver regeneration.
The Journal of biological chemistry. 274(8):4513-20 [PMID] 9988683.
1998
Abnormal regulation of the Ke 6 gene, a new 17beta-hydroxysteroid dehydrogenase in the cpk mouse kidney.
Molecular and cellular endocrinology. 143(1-2):9-22 [PMID] 9806346.
1998
Renal medullary rim sign in 2 adult quarter horses.
The Canadian veterinary journal = La revue veterinaire canadienne. 39(10):647-9 [PMID] 9789678.
1998
Ultrasound-assisted diagnosis of renal dysplasia in a 3-month-old Quarter Horse colt.
Veterinary radiology & ultrasound : the official journal of the American College of Veterinary Radiology and the International Veterinary Radiology Association. 39(2):143-6 [PMID] 9548143.
1996
[Osteocartilaginous reconstruction, research and clinical application].
Swiss surgery = Schweizer Chirurgie = Chirurgie suisse = Chirurgia svizzera. 2(6):264-8 [PMID] 8998637.
1996
Halothane resistance in Drosophila melanogaster: development of a model and gene localization techniques.
Anesthesia and analgesia. 83(1):147-55 [PMID] 8659726.

Education

Postdoctoral Fellow/Pharmacology & Experimental Neuroscience (T32 trainee in Neurovirology)
2008 · University of Nebraska Medical Center, Omaha NE
Postdoctoral Fellow/Pharmacology & Physiology (T32 Trainee in the pharmacology of drugs of abuse )
2005 · University of Rochester School of Medicine and Dentistry, Rochester, NY
Ph.D/Immunology, Microbiology & Virology (IMV cluster) and Neuroscience
2004 · University of Rochester School of Medicine and Dentistry, Rochester, NY
M.S/Microbiology & Immunology
2001 · University of Rochester School of Medicine and Dentistry, Rochester NY
B.S/Biotechnology-Genetic Engineering
1998 · Rochester Institute of Technology, Rochester NY

Contact Details

Emails:
Addresses:
Business Mailing:
PO Box 100275
GAINESVILLE FL 32610
Business Street:
1600 SW ARCHER RD
GAINESVILLE FL 32610