Jing Chen

Jing Chen,

Associate Scientist

Department: MD-PATHOLOGY-GENERAL
Business Phone: (352) 273-9262
Business Email: jic24@pathology.ufl.edu

Teaching Profile

Courses Taught
2021,2023-2024
GMS6622 Mitochondrial Biology in Aging and Disease

Research Profile

Areas of Interest
  • Autoimmune Disease
  • Diabetes
  • Genetics
  • Immunology of Type 1 diabetes
  • Immunometabolism
Open Researcher and Contributor ID (ORCID)

0000-0001-8008-8062

Publications

2024
NADPH Oxidase 2–Derived Reactive Oxygen Species Promote CD8+ T Cell Effector Function
The Journal of Immunology. 212(2):258-270 [DOI] 10.4049/jimmunol.2200691. [PMID] 38079221.
2024
Proteome-scale tissue mapping using mass spectrometry based on label-free and multiplexed workflows
Molecular & Cellular Proteomics. [DOI] 10.1016/j.mcpro.2024.100841.
2023
Proteome mapping of the human pancreatic islet microenvironment reveals endocrine-exocrine signaling sphere of influence.
Molecular & cellular proteomics : MCP. 22(8) [DOI] 10.1016/j.mcpro.2023.100592. [PMID] 37328065.
2022
Intergenomic and epistatic interactions control free radical mediated pancreatic β-cell damage.
Frontiers in genetics. 13 [DOI] 10.3389/fgene.2022.994501. [PMID] 36276935.
2021
Mitochondrial haplogroup G is associated with nonalcoholic fatty liver disease, while haplogroup A mitigates the effects of PNPLA3
Endocrinology, Diabetes & Metabolism. 4(1) [DOI] 10.1002/edm2.187. [PMID] 33532620.
2021
Use of Induced Pluripotent Stem Cells to Build Isogenic Systems and Investigate Type 1 Diabetes
Frontiers in Endocrinology. 12 [DOI] 10.3389/fendo.2021.737276. [PMID] 34858326.
2019
Neutrophil Cytosolic Factor 1 in Dendritic Cells Promotes Autoreactive CD8+ T Cell Activation via Cross-Presentation in Type 1 Diabetes
Frontiers in Immunology. 10 [DOI] 10.3389/fimmu.2019.00952. [PMID] 31118934.
2018
ACTH-independent Cushing’s syndrome with bilateral cortisol-secreting adrenal adenomas: a case report and review of literatures.
BMC endocrine disorders. 18(1) [DOI] 10.1186/s12902-018-0250-6. [PMID] 29685132.
2018
Mitochondrial Reactive Oxygen Species and Type 1 Diabetes.
Antioxidants & redox signaling. 29(14):1361-1372 [DOI] 10.1089/ars.2017.7346. [PMID] 29295631.
2018
Nanodroplet processing platform for deep and quantitative proteome profiling of 10–100 mammalian cells
Nature Communications. 9(1) [DOI] 10.1038/s41467-018-03367-w. [PMID] 29491378.
2018
Nanowell-mediated two-dimensional liquid chromatography enables deep proteome profiling of
Chemical science. 9(34):6944-6951 [DOI] 10.1039/c8sc02680g. [PMID] 30210768.
2017
T cells display mitochondria hyperpolarization in human type 1 diabetes
Scientific Reports. 7(1) [DOI] 10.1038/s41598-017-11056-9. [PMID] 28883439.
2017
The Type 1 Diabetes–Resistance Locus Idd22 Controls Trafficking of Autoreactive CTLs into the Pancreatic Islets of NOD Mice
The Journal of Immunology. 199(12):3991-4000 [DOI] 10.4049/jimmunol.1602037. [PMID] 29109122.
2015
Association of the mt-ND2 5178A/C polymorphism with Parkinson’s disease.
Neuroscience letters. 587:98-101 [DOI] 10.1016/j.neulet.2014.12.005. [PMID] 25511548.
2015
Distinct differences in the responses of the human pancreatic β-cell line EndoC-βH1 and human islets to proinflammatory cytokines.
American journal of physiology. Regulatory, integrative and comparative physiology. 309(5):R525-34 [DOI] 10.1152/ajpregu.00544.2014. [PMID] 26084699.
2015
Liquid Chromatography-Mass Spectrometry Metabolic and Lipidomic Sample Preparation Workflow for Suspension-Cultured Mammalian Cells using Jurkat T lymphocyte Cells.
Journal of proteomics & bioinformatics. 8(6):126-132 [PMID] 26401069.
2015
Respiration and substrate transport rates as well as reactive oxygen species production distinguish mitochondria from brain and liver.
BMC biochemistry. 16 [DOI] 10.1186/s12858-015-0051-8. [PMID] 26358560.
2014
Use of chemical probes to detect mitochondrial ROS by flow cytometry and spectrofluorometry.
Methods in enzymology. 542:223-41 [DOI] 10.1016/B978-0-12-416618-9.00012-1. [PMID] 24862269.
2012
Immune-mediated β-cell death in type 1 diabetes: lessons from human β-cell lines.
European journal of clinical investigation. 42(11):1244-51 [DOI] 10.1111/j.1365-2362.2012.02711.x. [PMID] 22924552.
2012
Oxidative stress and beta cell dysfunction.
Methods in molecular biology (Clifton, N.J.). 900:347-62 [DOI] 10.1007/978-1-60761-720-4_17. [PMID] 22933078.
2011
mt-Nd2(a) Modifies resistance against autoimmune type 1 diabetes in NOD mice at the level of the pancreatic β-cell.
Diabetes. 60(1):355-9 [DOI] 10.2337/db10-1241. [PMID] 20980458.
2011
Role of genetics in resistance to type 1 diabetes.
Diabetes/metabolism research and reviews. 27(8):849-53 [DOI] 10.1002/dmrr.1260. [PMID] 22069272.
2011
Role of the mitochondria in immune-mediated apoptotic death of the human pancreatic β cell line βLox5.
PloS one. 6(6) [DOI] 10.1371/journal.pone.0020617. [PMID] 21738580.
2011
Superoxide production by macrophages and T cells is critical for the induction of autoreactivity and type 1 diabetes.
Diabetes. 60(8):2144-51 [DOI] 10.2337/db10-1222. [PMID] 21715554.
2009
Chapter 24 Quantification, localization, and tissue specificities of mouse mitochondrial reactive oxygen species production.
Methods in enzymology. 456:439-57 [DOI] 10.1016/S0076-6879(08)04424-8. [PMID] 19348903.
2009
Rosiglitazone inhibits RANTES expression by human epidermal keratinocytes.
European journal of dermatology : EJD. 19(2):176-7 [DOI] 10.1684/ejd.2008.0591. [PMID] 19106050.
2008
Commonalities of genetic resistance to spontaneous autoimmune and free radical–mediated diabetes
Free Radical Biology and Medicine. 45(9):1263-1270 [DOI] 10.1016/j.freeradbiomed.2008.07.020. [PMID] 18718526.
2008
Role of increased ROS dissipation in prevention of T1D.
Annals of the New York Academy of Sciences. 1150:157-66 [DOI] 10.1196/annals.1447.045. [PMID] 19120287.
2006
CD38 is required for the peripheral survival of immunotolerogenic CD4+ invariant NK T cells in nonobese diabetic mice.
Journal of immunology (Baltimore, Md. : 1950). 177(5):2939-47 [PMID] 16920929.
2006
Mitochondrial complex I activity is significantly decreased in a patient with maternally inherited type 2 diabetes mellitus and hypertrophic cardiomyopathy associated with mitochondrial DNA C3310T mutation: a cybrid study.
Diabetes research and clinical practice. 74(2):148-53 [PMID] 16828917.
2006
Novel Leptin Receptor Mutation in NOD/LtJ Mice Suppresses Type 1 Diabetes Progression: II. Immunologic Analysis
Diabetes. 55:171-178
2006
Targeted disruption of CD38 accelerates autoimmune diabetes in NOD/Lt mice by enhancing autoimmunity in an ADP-ribosyltransferase 2-dependent fashion.
Journal of immunology (Baltimore, Md. : 1950). 176(8):4590-9 [PMID] 16585549.
2005
“Agouti NOD”: identification of a CBA-derived Idd locus on Chromosome 7 and its use for chimera production with NOD embryonic stem cells.
Mammalian genome : official journal of the International Mammalian Genome Society. 16(10):775-83 [PMID] 16261419.
2000
A patient with type 2 diabetes mellitus associated with mutations in calcium sensing receptor gene and mitochondrial DNA.
Biochemical and biophysical research communications. 278(3):808-13 [PMID] 11095989.
2000
Disruption of aldose reductase gene (Akr1b1) causes defect in urinary concentrating ability and divalent cation homeostasis
Biochemical and Biophysical Research Communications. 277(2):281-286

Grants

Feb 2023 ACTIVE
Discovery and Roles of In Situ Islet Neoantigens in Human Type 1 Diabetes
Role: Co-Investigator
Funding: NATL INST OF HLTH NIDDK
Oct 2020 ACTIVE
Multi-omic 3D tissue maps for a Human BioMolecular Atlas
Role: Co-Investigator
Funding: PACIFIC NW NATL LAB via NATL INST OF HLTH NIDDK
Feb 2020 – Jun 2024
Pathways and Critical Regulators of Early Beta-cell Dysfunction in Type 1 Diabetes
Role: Faculty
Funding: BATTELLE MEMORIAL INST via NATL INST OF HLTH NIDDK
Aug 2019 – Jul 2021
Engineering a Human Microphysiological System for the Characterization of Islet-Immune Interactions
Role: Co-Investigator
Funding: NATL INST OF HLTH NIDDK
Jan 2019 – Jan 2022
The Role of IFN? supplementation in T cell effector function in Patients with Chronic Granulomatous Disease
Role: Co-Investigator
Funding: UNIV OF SOUTH FLORIDA via HORIZON THERAPEUTICS
Jun 2018 – May 2023
Immune Function and the Progression to Type 1 Diabetes
Role: Co-Investigator
Funding: NATL INST OF HLTH NIAID
Apr 2017 – Mar 2021
Advanced Liver 3-D Tissue Models for Drug Development Applications to Support Toxicology, Drug Metabolism, Target ID & Pharmacology
Role: Faculty
Funding: ADV REGENERATIVE MANUFACTURING INST via US ARMY RESEARCH LABORATORY
Jan 2015 – Aug 2018
Regulatory Networks and Biomarkers of Beta-cell Dysfunction and Apoptosis
Role: Co-Investigator
Funding: PACIFIC NW NATL LAB via NATL INST OF HLTH NIDDK

Contact Details

Phones:
Business:
(352) 273-9262
Emails:
Addresses:
Business Mailing:
1275 CENTER DR J587
GAINESVILLE FL 32610
Business Street:
BIOMEDICAL SCIENCE BUILDING
BIOMEDICAL SCIENCE BUILDING
ROOM J587
GAINESVILLE FL 326100001